Construction of minimally-sheltered knockouts mutants of Neurospora crassa
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چکیده
منابع مشابه
Mutants of NEUROSPORA CRASSA Permeable to Histidinol.
I N Neurospora, L-histidinol is the immediate precursor of L-histidine, as shown by the isolation of L-histidinol dehydrogenase and the study of its catalytic functions (AMES 1957; CREASER, BENNETT and DRYSDALE 1965). The failure of histidine mutants, with the histidinol dehydrogenase function intact, to grow on histidinol, suggests that histidinol cannot enter the cell. However, CATCHESIDE ( 1...
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ADIATION-SENSITIVE mutants in bacteria have been reported repeatedly in recent years (s,ee SETLOW 1966). Several loci concerned with sensitivity have been mapped. Some of these mutants not only lacked the ability to repair damaged DNA but also failed to undergo genetic recombination (CLARK and MARGULIES 1965; HOWARD-FLANDERS and THERIOT 1966; VAN DE PUTTE et al. 1966). Among the eucaryotic orga...
متن کاملMutants affecting thymidine metabolism in Neurospora crassa.
When (14)C-thymidine labeled only in the ring is administered to Neurospora crassa, the majority of the recovered label is found in the ribonucleic acid (RNA). Three mutants were isolated in which different steps are blocked in the pathway that converts the pyrimidine ring of thymidine to an RNA precursor. Evidence from genetic, nutritional, and accumulation studies with the three mutants shows...
متن کاملBiotransformation of Hydrocortisone by Neurospora crassa
The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...
متن کاملIsolation of circadian clock mutants of Neurospora crassa.
Three mutants of Neurospora crassa have been isolated which have altered period lengths of their circadian rhythm of conidiation. The strains, designated "frequency" (frq), were obtained after mutagenesis of the band (bd) strain with N-methyl-N'-nitro-N-nitrosoguanidine. In continuous darkness at 25 degrees bd has a period length of 21.6 +/- 0.5 hours; under the same conditions the period lengt...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 2005
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1123